Question 1 Report
| Row | First | Middle | Last |
|---|---|---|---|
| A | cut gene from human DNA | seal gene into plasmid with ligase | bacterium expresses gene |
| B | bacterium expresses gene | cut gene from DNA | seal into plasmid |
| C | seal into plasmid | bacterium expresses gene | cut gene from DNA |
| D | bacterium reproduces | cut gene from DNA | seal into plasmid |
The table shows stages of inserting a human gene into bacteria. Which row lists the stages in the correct sequence from first to last?
The stages must be ordered from what physically comes first to what comes last. The correct row runs cut the gene from human DNA, then seal it into the plasmid with ligase, then the bacterium expresses the gene. Expression, the making of the protein, is the final outcome, so any row that has the bacterium expressing the gene first, or that seals the gene into a plasmid before the gene has been cut out, breaks the required cause-and-effect order.
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