Row First Middle Last A cut gene from human DNA seal gene into plasmid with ligase bacterium expresses gene B bacterium expresses gene cut gene from DNA sea...

Assessment: Biology 0610 | Paper 2 Mock 01 | Multiple Choice (Extended) Subject: Biology - 0610

Question 1 Report

RowFirstMiddleLast
Acut gene from human DNAseal gene into plasmid with ligasebacterium expresses gene
Bbacterium expresses genecut gene from DNAseal into plasmid
Cseal into plasmidbacterium expresses genecut gene from DNA
Dbacterium reproducescut gene from DNAseal into plasmid

The table shows stages of inserting a human gene into bacteria. Which row lists the stages in the correct sequence from first to last?

Answer Details

The stages must be ordered from what physically comes first to what comes last. The correct row runs cut the gene from human DNA, then seal it into the plasmid with ligase, then the bacterium expresses the gene. Expression, the making of the protein, is the final outcome, so any row that has the bacterium expressing the gene first, or that seals the gene into a plasmid before the gene has been cut out, breaks the required cause-and-effect order.

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