Substrate concentration Relative rate low 5 medium 9 high 12 very high 12 The table shows reaction rate at increasing substrate concentrations with a fixed ...
Question 1 Report
| Substrate concentration | Relative rate |
|---|---|
| low | 5 |
| medium | 9 |
| high | 12 |
| very high | 12 |
The table shows reaction rate at increasing substrate concentrations with a fixed amount of enzyme. Why does the rate stop increasing at high concentrations?
Answer Details
With a fixed amount of enzyme there is a limited number of active sites. As substrate concentration rises the rate increases until every active site is occupied at any moment; beyond that point (rate steady at 12) extra substrate cannot be used, so the rate stops rising. This plateau is not due to a temperature or pH change or to denaturation, but to all the active sites being fully occupied.
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