A potometer is a piece of apparatus used to measure the rate at which a leafy shoot takes up water. A student set up the potometer shown in Fig. 3.1 and use...

Assessment: Biology 0610 | Paper 3 Mock 01 | Theory (Core) Subject: Biology - 0610

Question 1 Report

A potometer is a piece of apparatus used to measure the rate at which a leafy shoot takes up water. A student set up the potometer shown in Fig. 3.1 and used it to measure the rate of water uptake by a leafy shoot under three different conditions. As the shoot took up water, an air bubble moved along a scale. Table 3.1 shows the rate of water uptake that was measured under each of the three conditions.

diagram

Fig. 3.1

Table 3.1

Condition (air, temperature / C)Rate of water uptake / mm per minute
still air, 2012
moving air, 2026
moving air, 3038

(a) Using Table 3.1, state the condition that gave the highest rate of water uptake. [1]
(b) Explain why moving air increased the rate of water uptake. [3]
(c) Explain why the higher temperature increased the rate of water uptake. [2]
(d) Explain how the movement of the air bubble along the scale allows the rate of water uptake to be found. [2]
(e) The volume of water taken up is slightly greater than the true water loss from the shoot. Suggest one reason for this difference. [1]
(f) Suggest one variable that must be kept constant for the comparison between the conditions to be fair. [2]

Answer Details

This question tests the potometer and the factors that affect transpiration and therefore water uptake. Faster evaporation from the leaves keeps the diffusion gradient of water vapour steep, so water is pulled up faster.

(a) [1] The highest rate is moving air at 30 °C (38 mm per minute) [1].

(b) [3] Moving air blows away the water vapour that collects around the stomata [1]; this keeps the water-vapour concentration (water potential) gradient between the leaf and the air steep [1]; so transpiration, and therefore water uptake, is faster [1].

(c) [2] A higher temperature gives the water molecules more kinetic energy, so evaporation from the leaf is faster [1]; this speeds up transpiration and so water uptake [1].

(d) [2] As the shoot takes up water, the air bubble moves along the scale [1]; measuring the distance the bubble moves in a known time gives the rate of uptake (e.g. mm per minute) [1].

(e) [1] Uptake slightly exceeds loss because some of the water taken up is used by the cells (for photosynthesis) or retained in the shoot rather than being lost as vapour [1].

(f) [2] Name one variable and say why it matters, e.g. light intensity must be kept constant [1] because it changes how far the stomata open and so alters transpiration, which would make the comparison unfair [1] (accept: the same shoot/leaf area; humidity).

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