Which of the following breeding practices would lead to reduced hybrid vigour

Inbreeding is a breeding practice that leads to reduced hybrid vigor. When individuals who are closely related are bred together, the offspring can inherit two copies of the same gene, which increases the likelihood of inheriting harmful genetic traits. This can lead to reduced genetic diversity, weaker immune systems, and other health problems. Outbreeding, crossbreeding, and line breeding all involve breeding individuals who are less closely related, which can increase genetic diversity and strengthen desirable traits. Therefore, inbreeding is the practice that would lead to reduced hybrid vigor.