Which of the following did not take place on the day of Pentecost?

The Holy Spirit did not say "set apart Paul and Barnabas" on the day of Pentecost. On the day of Pentecost, the Holy Spirit descended upon the apostles and they were filled with the Holy Spirit. They began to speak in different tongues, and a great wind filled the room where they were gathered. Tongues of fire also rested upon their heads. This event marked the birth of the Christian church, and it is celebrated annually as the Feast of Pentecost. Therefore, the correct answer is "The Holy Spirit said 'set apart Paul and Barnabas'."