A technician is comparing the action of amylase at different temperatures before making a medicine for patients whose pancreas produces too little enzyme. F...

Assessment: Combined Science Double Award 9204 | Paper 1 Mock 01 | Biology Subject: Combined Science Double Award - 9204

Question 1 Report

9204-p1-digestion-2-amylase-waterbath

A technician is comparing the action of amylase at different temperatures before making a medicine for patients whose pancreas produces too little enzyme. Fig. 1 shows the apparatus for one test tube. Equal volumes of starch suspension and amylase are mixed, then drops are removed onto iodine in a spotting tile. The water bath keeps the temperature constant. A student records the time when the iodine no longer becomes blue-black. All test tubes contain the same pH buffer and the same volume of enzyme.

(a) Identify the food substance broken down by amylase. [1]
(b) Explain why the technician uses a water bath. [2]
(c) Use the iodine result to identify when all the starch has been digested. [1]
(d) Calculate the mean time if three readings are 42 s, 46 s and 44 s. [2]

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