The sentence is comparing the manners of two people - "his" and "his wife's". The first part of the sentence says that "his manners are uncouth", which means that they are lacking in refinement, elegance, or polish. The word "while" indicates a contrast, so the second part of the sentence implies that "his wife's" manners are different from "his" - they are "refined". "Refined" means elegant, cultured, and polite, so it is the correct answer.