Which of these pair of substances must be present for a seed to germinate in a laboratory set-up?

For a seed to germinate in a laboratory set-up, the key pair of substances required are heat and water.

Water is essential because it activates the enzymes that begin the germination process. When a seed absorbs water, it swells and breaks the seed coat. This process is known as imbibition, and it is the first step in germination. The absorbed water allows the enzymes to start breaking down stored food resources within the seed, providing the energy necessary for the growth of the embryonic plant.

Heat, on the other hand, is important because most seeds need to be within a certain temperature range to germinate effectively. Appropriate warmth can facilitate enzymatic activities and biochemical processes needed for growth. The required temperature varies between species, but generally, seeds need warmth to sprout successfully.

While microbes can contribute to soil fertility and the decomposition of organic material, they are not directly necessary for the germination process of seeds, nor is soil required in a controlled laboratory environment.

Similarly, while manure can provide nutrients in an outdoor setting, it is not a vital component in the controlled germination process in a lab. The focus in such controlled experiments is typically on the primary resources that directly aid in the seed's initial growth, namely water and suitable temperature from heat.