Genetic Modification
Akopọ
Genetic modification lets scientists do something nature never could: move a single chosen gene from one species into a completely different one. A human gene placed in a bacterium turns that bacterium into a living factory for a human protein. It is one of the most powerful, and most debated, tools in biology.
In this lesson you will define genetic modification precisely, then (for [Supplement]) follow the step-by-step process of using bacteria to make a human protein: cutting out the gene with restriction enzymes, splicing it into a plasmid with ligase, and growing the modified bacteria so they express the human gene.
Awọn Afojusun
- Describe genetic modification as changing the genetic material of an organism by removing, changing or inserting individual genes
- [Supplement] Outline the process of genetic modification using bacterial production of a human protein as an example, limited to: (a) isolation of the DNA making up a human gene using restriction enzymes, forming sticky ends (b) cutting of bacterial plasmid DNA with the same restriction enzymes, forming complementary sticky ends (c) insertion of human DNA into bacterial plasmid DNA using DNA ligase to form a recombinant plasmid (d) insertion of recombinant plasmids into bacteria (specific details are not required) (e) multiplication of bacteria containing recombinant plasmids (f) expression in bacteria of the human gene to make the human protein
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Ìdánwò Ẹ̀kọ́
Oriire fun ipari ẹkọ lori Genetic Modification. Ni bayi ti o ti ṣawari naa awọn imọran bọtini ati awọn imọran, o to akoko lati fi imọ rẹ si idanwo. Ẹka yii nfunni ni ọpọlọpọ awọn adaṣe awọn ibeere ti a ṣe lati fun oye rẹ lokun ati ṣe iranlọwọ fun ọ lati ṣe iwọn oye ohun elo naa.
Iwọ yoo pade adalu awọn iru ibeere, pẹlu awọn ibeere olumulo pupọ, awọn ibeere idahun kukuru, ati awọn ibeere iwe kikọ. Gbogbo ibeere kọọkan ni a ṣe pẹlu iṣaro lati ṣe ayẹwo awọn ẹya oriṣiriṣi ti imọ rẹ ati awọn ogbon ironu pataki.
Lo ise abala yii gege bi anfaani lati mu oye re lori koko-ọrọ naa lagbara ati lati ṣe idanimọ eyikeyi agbegbe ti o le nilo afikun ikẹkọ. Maṣe jẹ ki awọn italaya eyikeyi ti o ba pade da ọ lójú; dipo, wo wọn gẹgẹ bi awọn anfaani fun idagbasoke ati ilọsiwaju.
- Genetic modification is best defined as: A. breeding the best individuals over generations B. changing the genetic material by removing, changing or inserting genes C. cloning an entire organism D. exposing cells to sunlight Answer: B
- [Supplement] Restriction enzymes are used to: A. join DNA pieces together B. cut DNA at specific sites, leaving sticky ends C. copy whole cells D. make proteins Answer: B
- [Supplement] The enzyme that joins a gene into a plasmid is: A. a restriction enzyme B. DNA ligase C. amylase D. pectinase Answer: B
- [Supplement] A plasmid carrying an inserted gene is called a: A. chromosome B. recombinant plasmid C. ribosome D. vector enzyme Answer: B
- [Supplement] The human gene and the plasmid are cut with the same restriction enzyme so that: A. the bacteria die B. their sticky ends are complementary and can join C. the gene is destroyed D. no ligase is needed Answer: B
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Biotechnology
Describe gel electrophoresis
"Biology: Biotechnology" by OpenStax, OpenStax, Rice University, licensed under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Source: https://openstax.org/books/biology/pages/17-1-biotechnology. Access for free at https://openstax.org/books/biology/pages/1-introduction
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Ṣe Adaṣe Awọn Ibeere Idanwo Adaṣe
Ṣe o fẹ ṣe adaṣe awọn ibeere idanwo adaṣe nipa Genetic Modification? Ṣe igbasilẹ ohun elo Green Bridge CBT lati wọle si awọn ibeere idanwo adaṣe ati awọn ayẹwo adaṣe kikun fun koko-ọrọ yii.
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